Poster De Conférence Année : 2014

Contribution of phosphatases to adaptation of rhizobial symbiosis with legumes to phosphorus deficiency

Résumé

To understand the mechanisms used by legumes to improve their phosphorus use efficiency (PUE) for symbiotic nitrogen fixation under P-deficiency, an in situ RT-PCR methodology was used to localize and quantify the transcripts of candidate phosphatases genes in nodules of two common bean RILs 115 and 147 in hydro-aeroponic culture under deficient versus sufficient P supply. Our findings have revealed that expression of phytase and fructose 1,6 bisphosphatase (FBPase) genes and activities of the corresponding enzymes were positively correlated with increases both of the rhizobial symbiosis efficiency in use of P for N2 fixation and nodule O2 permeability. Under P-deficiency, this positive correlation was more significant for the P-efficient RIL115 than for the P-inefficient RIL147. It is concluded that these phosphatases play a role in adaptation to P-deficiency and are likely involved in nodule respiration linked to symbiotic nitrogen fixation.

Fichier non déposé

Dates et versions

hal-01268835 , version 1 (04-02-2016)

Identifiants

  • HAL Id : hal-01268835 , version 1
  • PRODINRA : 280518

Citer

Mohamed Lazali, Laurie Amenc, Chahinez Benadis, Samira Brahimi, Josiane Abadie, et al.. Contribution of phosphatases to adaptation of rhizobial symbiosis with legumes to phosphorus deficiency. PSP5 2014 - Facing Phosphorus Scarcity, Aug 2014, Montpellier, France. 2014. ⟨hal-01268835⟩
483 Consultations
0 Téléchargements

Partager

  • More