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Two-Dimensional Liquid Chromatography


Two-dimensional liquid chromatography saw significant developments in handling the need to separate a rising number of highly complex samples. 2D-LC is the coupling of the separation of a complex sample by a first LC method to a second different LC method. This chapter details the different ways to perform such coupling 2D-LC methods: heart-cutting method; offline, online, and stop-flow methods, and comprehensive LC × LC. The critical parameter is peak capacity that is defined and calculated in classical LC and 2D-LC methods. It is linked to the orthogonality of the separation power of the two coupled LC methods. From a practical point of view, the effective peak capacity is compared with the theoretical one. Analysis time and dilution are also two critical parameters in 2D-LC that need to be considered. All parameters are presented describing a practical method development.
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hal-01255456 , version 1 (13-01-2016)



Morgan Sarrut, Nicola Marchetti, Sabine Heinisch. Two-Dimensional Liquid Chromatography. Anderson J. L., Berthod A., Pino Estévez V., Stalcup A. M. Analytical Separation Science, 4, Wiley-VCH Verlag GmbH & Co. KGaA., pp.1357-1384, 2015, 9783527678129. ⟨10.1002/9783527678129.assep049⟩. ⟨hal-01255456⟩
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