The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Clinical Microbiology and Infectious Diseases Année : 2015

The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae

R. N. Tan
  • Fonction : Auteur
C. Hennequin
L. Gibold
  • Fonction : Auteur
F. Robin

Résumé

The MastA (R) D68C test is a phenotypical test that allows the detection of extended-spectrum beta-lactamase (ESBL) production, even in AmpC-producing Enterobacteriaceae. We assessed its detection accuracy against a large collection of 106 Enterobacteriaceae isolates producing a wide diversity of well-characterized beta-lactamases (53 ESBL producers, 25 Amp. producers, seven AmpC and ESBL producers, five carbapenemase producers, three carbapenemase and ESBL producers, one AmpC, carbapenemase, and ESBL producer, three TEM-1 producers, three SHV-1 producers, three OXA-1 producers, and one hyperOXY producer, ATCC 35218, ATCC 25922 [a beta-lactamase-negative control strain]). The results were compared with those of the double disk test and the Clinical and Laboratory Standards Institute (CLSI) confirmatory test for the detection of ESBL. The sensitivity was 90.6 % for the synergy test, 87.5 % for the CLSI method, and only 73.1 % for D68C, which, however, reached 92.1 % if the strains for which supplementary investigations were recommended and the complex mutant TEM (CMT)-producing strains were excluded versus 94.1 % and 88.2 % for the other methods. The specificity was 90.2 % for the synergy test and 100 % for the CLSI method and D68C. D68C was also efficient in detecting AmpC-overproducing strains (sensitivity = 97 %, specificity = 95.9 %): among the 74 strains belonging to natural AmpC-producing species, the sensitivity and specificity were 100 and 94.8 %, respectively. The MastA (R) D68C-test is a promising method that is easy to perform for the detection of current ESBLs and could also be useful for the detection of plasmid-encoded AmpC enzymes (sensitivity = 100 %).
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Dates et versions

hal-01247494 , version 1 (22-12-2015)

Identifiants

Citer

C. Nourrisson, R. N. Tan, C. Hennequin, L. Gibold, R. Bonnet, et al.. The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. European Journal of Clinical Microbiology and Infectious Diseases, 2015, 34 (5), pp.975-983. ⟨10.1007/s10096-014-2305-6⟩. ⟨hal-01247494⟩
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