Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity - Archive ouverte HAL
Article Dans Une Revue PLoS ONE Année : 2013

Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity

Kelly Aubertin
Stéphanie Bonneau
Amanda K. A. Silva
Claire Wilhelm

Résumé

The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations (cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.
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Dates et versions

hal-01244562 , version 1 (19-11-2016)

Identifiants

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Kelly Aubertin, Stéphanie Bonneau, Amanda K. A. Silva, Jean-Claude Bacri, François Gallet, et al.. Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity. PLoS ONE, 2013, 8 (12), ⟨10.1371/journal.pone.0084850⟩. ⟨hal-01244562⟩
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