Progesterone plays a critical role in canine oocyte maturation and fertilization - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of Reproduction Année : 2015

Progesterone plays a critical role in canine oocyte maturation and fertilization

Résumé

Canine oocyte maturation and fertilization take place within the oviducts under increasing plasma levels of progesterone (P4). In order to investigate the role of P4 in these processes, 51 Beagle bitches were treated with the P4 receptor antagonist aglepristone at the end of proestrus and 32 females were kept untreated. Fifteen treated and 13 control bitches were inseminated at Days +1 and +2 after ovulation (Day 0). Stages of oocyte maturation and embryo development were determined after ovariectomy at different time points after ovulation. Aglepristone did not prevent ovulation but delayed the resumption of oocyte meiosis and inhibited its progression: first metaphase (M) I stages were observed at 173 h post-ovulation and 39% of oocytes reached MII as late as 335 h post-ovulation in treated females whereas first MI occurred at 76 h and 100% of oocytes were in MII at 109 h post-ovulation in controls. Aglepristone extended the stay of morphologically normal oocytes within the oviducts: first signs of oocyte degeneration were observed at 335 h in treated vs. 100-110 h post-ovulation in control bitches. In inseminated females, aglepristone prevented sperm progression toward the oviducts and fertilization, although motile spermatozoa were observed in the uterine tip flush and within the cranial uterine glands. A proteomic analysis of the tubal fluid from treated and control non inseminated bitches at Day +4 evidenced 79 differential proteins potentially involved in the oocyte phenotype. In conclusion, P4 plays key roles in post-ovulatory canine oocyte maturation, aging and in fertilization.

Domaines

Autre [q-bio.OT]

Dates et versions

hal-01225259 , version 1 (05-11-2015)

Identifiants

Citer

Karine Reynaud, Marie Saint-Dizier, Muhammad Zahid Tahir, Tiphaine Havard, Grégoire Harichaux, et al.. Progesterone plays a critical role in canine oocyte maturation and fertilization. Biology of Reproduction, 2015, 93 (4), ⟨10.1095/biolreprod.115.130955⟩. ⟨hal-01225259⟩
233 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More