In this review, we comprehensively summarize the synthetic approach that can be developed for the design and the preparation of iron complex intercalated LDH structure which mainly involves oxalate, citrate, ferrocyanide, ferrocene, N,N’-ethylenebis(salycylideneiminato) (salen), porphyrine and proteins. Then, we propose a detailed overview of the characterization tools allowing a deep insight into the iron complex intercalated LDH. Finally, the potential applications reported in the literature are also considered highlighting the huge potential of such kind of iron based nanostructured layered materials.