Progress and challenges in pedigree-based QTL analysis utilizing high density marker data on related full sib families: A case study on fruit firmness in apple. - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2014

Progress and challenges in pedigree-based QTL analysis utilizing high density marker data on related full sib families: A case study on fruit firmness in apple.

Marco C. A. M. Bink
  • Fonction : Auteur
Johannes Kruisselbrink
  • Fonction : Auteur
Hans Jansen
  • Fonction : Auteur
Roeland Voorrips
  • Fonction : Auteur
Sara Longhi
Mario Di Guardo
  • Fonction : Auteur
F. Costa
  • Fonction : Auteur
Eric van de Weg
  • Fonction : Auteur

Résumé

The power to identify, map and quantify QTL underlying complex traits may substantially increase when considering multiple segregating progenies simultaneously. The relatedness among these progenies can be fully exploited when the pedigree is known and the ancestors are available for genotyping. Previously, we successfully used the lexQTL software to map QTL in such a Pedigree Based Analysis (PBA) approach to a large pedigreed population in apple with only 87 SSR markers (Bink et al. 2014). The current availability of high-throughput SNP genotyping infrastructures allows marker genotyping at much higher densities, which will contribute to higher mapping resolution and more accurate QTL characterization but also poses challenges to the QTL mapping software. Within the framework of the projects SCRI-RosBREED (#2009-51181-05808) and EU-FP7 FruitBreedomics (#FP7- 65582), we have improved and extended functionality of the FlexQTL software to better handle large numbers of SNP markers with regard to computation time, the phasing of the lowly-informative SNPs in complex pedigrees (e.g., inbreeding loops). Furthermore visualization tools were added to inspect recombination events. Furthermore, highly informative multi-allelic haplotypes can now be built comprising consecutive low informative SNPs, which will reduce computational effort without losing information from the high density marker data. The progress and challenges on utilizing high density SNP data in Rosaceae crops will be highlighted via a case study, i.e., applying the new version of FlexQTL software to fruit firmness trait on a large 20K genotyped apple population as available from the FruitBreedomics project.
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Dates et versions

hal-01209968 , version 1 (02-10-2015)

Identifiants

  • HAL Id : hal-01209968 , version 1
  • PRODINRA : 268194

Citer

Marco C. A. M. Bink, Johannes Kruisselbrink, Hans Jansen, Roeland Voorrips, Amy Iezzoni, et al.. Progress and challenges in pedigree-based QTL analysis utilizing high density marker data on related full sib families: A case study on fruit firmness in apple.. 7. International Rosaceae Genomics Conference, Jun 2014, Seattle, United States. , 2014. ⟨hal-01209968⟩
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