Generally, <30% of dairy cattle’s nitrogen intake is retained in milk. Large amounts of nitrogen are excreted in manure, especially in urine, with damaging impacts on the environment. This study explores the effect of lowering dietary degradable nitrogen supplies – while maintaining metabolisable protein – on dairy cows’ performance, nitrogen use efficiency and gas emissions (NH3, N2O, CH4) at barn level with tied animals. Two dietary N concentrations (CP: 12% DM for LowN; 18% DM for HighN) were offered to two groups of three lactating dairy cows in a split-plot design over four periods of 2 weeks. Diets were formulated to provide similar metabolisable protein supply, with degradable N either in deficit or in excess (PDIN of 84 and 114 g/kg DM for LowN and HighN, respectively). Cows ingested 0.8 kg DM/day less on the LowN diet, which was also 2.5% less digestible. Milk yield and composition were not significantly affected. N exported in milk was 5% lower (LowN: 129 g N/day; HighN: 136 g N/day; P < 0.001) but milk protein yield was not significantly affected (LowN: 801 g/day; HighN: 823 g/day; P = 0.10). Cows logically ingested less nitrogen on the LowN diet (LowN: 415 g N/day; HighN: 626 g N/day; P < 0.001) resulting in a higher N use efficiency (N milk/N intake; LowN: 0.31; HighN: 0.22; P < 0.001). N excreted in urine was almost four times lower on the LowN diet (LowN: 65 g N/day; HighN: 243 g N/day; P < 0.001) while urinary urea N concentration was eightfold lower (LowN: 4.6 g/l; HighN: 22.9 g/l; P < 0.001). Ammonia emission (expressed in g/h in order to remove periods of the day with potential interferences with volatile molecules from feed) was also lower on the LowN diet (LowN: 1.03 g/h per cow; HighN: 1.25 g/h per cow; P < 0.05). Greenhouse gas emissions (N2O and CH4) at barn level were not significantly affected by the amount of dietary N. Offering low amounts of degradable protein with suitable metabolisable protein amounts to cattle improved nitrogen use efficiency and lowered ammonia emissions at barn level. This strategy would, however, need to be validated for longer periods, other housing systems (free stall barns) and at farm level including all stages of manure management.