Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2014

Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum

Résumé

Neonicotinoid insecticides act on nicotinic acetylcholine receptor and are particularly effective against sucking pests. They are widely used in crops protection to fight against aphids, which cause severe damage. In the present study we evaluated the susceptibility of the pea aphid Acyrthosiphon pisum to the commonly used neonicotinoid insecticides imidacloprid (IMI), thiamethoxam (TMX) and clothianidin (CLT). Binding studies on aphid membrane preparations revealed the existence of high and low-affinity binding sites for [H-3]-IMI (Kd of 0.16 +/- 0.04 nM and 41.7 +/- 5.9 nM) and for the nicotinic antagonist [I-125]-alpha- bungarotoxin (Kd of 0.008 +/- 0.002 nM and 1.135 +/- 0.213 nM). Competitive binding experiments demonstrated that TMX displayed a higher affinity than IMI for [I-125]-alpha-bungarotoxin binding sites while CLT affinity was similar for both [I-125]-alpha-bungarotoxin and [H-3]-IMI binding sites. Interestingly, toxicological studies revealed that at 48 h, IMI (LC50 = 0.038 mu g/ml) and TMX (LC50 = 0.034 mu g/ml) were more toxic than CLT (LC50 = 0.118 mu g/ml). The effect of TMX could be associated to its metabolite CLT as demonstrated by HPLC/MS analysis. In addition, we found that aphid larvae treated either with IMI, TMX or CLT showed a strong variation of nAChR subunit expression. Using semi-quantitative PCR experiments, we detected for all insecticides an increase of Apisum alpha 10 and Apisum beta 1 expressions levels, whereas Apisum beta 2 expression decreased. Moreover, some other receptor subunits seemed to be differently regulated according to the insecticide used. Finally, we also demonstrated that nAChR subunit expression differed during pea aphid development. Altogether these results highlight species specificity that should be taken into account in pest management strategies
Fichier principal
Vignette du fichier
2014_Taillebois_PlosOne_1.pdf (750.64 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01208711 , version 1 (27-05-2020)

Identifiants

Citer

Emiliane Taillebois, Abdelhamid Beloula, Sophie Quinchard, Stéphanie Jaubert-Possamai, Antoine Daguin, et al.. Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum. PLoS ONE, 2014, 9 (5), pp.e96669. ⟨10.1371/journal.pone.0096669⟩. ⟨hal-01208711⟩
122 Consultations
60 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More