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Article Dans Une Revue Molecular Reproduction and Development Année : 2012

Evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos

Résumé

c-Myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. In addition, the evolutionary history of c-myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in teleosts. To gain new insight into c-myc genes in teleosts, the present study was designed to clarify the evolutionary history of c-myc gene(s) in teleosts and to subsequently characterize DNA methylation and early embryonic expression patterns in a cyprinid fish. Our results show that a duplication of c-myc gene occurred before or around the teleost radiation, as a result of the teleost-specific whole genome duplication giving rise to c-myca and c-mycb in teleosts and was followed by a loss of the c-mycb gene in the Gasterosteiforms and Tetraodontiforms. Our data also demonstrate that both c-myc genes previously identified in carp and goldfish are co-orthologs of the zebrafish c-myca. These results indicate the presence of additional c-myca duplication in Cyprininae. We were able to identify differences between the expression patterns of the two goldfish c-myca genes in oocytes and early embryos. These differences suggest a partial sub-functionalization of c-myca genes after duplication. Despite differences in transcription patterns, both of the c-myca genes displayed similar DNA methylation patterns during early development and in gametes. Together, our results clarify the evolutionary history of the c-myc gene in teleosts and provide new insight into the involvement of c-myc in early embryonic development in cyprinids. Mol. Reprod. Dev. 79:8596, 2012. (C) 2011 Wiley Periodicals, Inc.

Dates et versions

hal-01205046 , version 1 (24-09-2015)

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Lucie Marandel, Catherine Labbé, Julien Bobe, Pierre-Yves Le Bail. Evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos. Molecular Reproduction and Development, 2012, 79 (2), pp.85-96. ⟨10.1002/mrd.22004⟩. ⟨hal-01205046⟩
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