Intestinal CD103+ dendritic cells are key players in the innate immune control of [i]Cryptosporidium parvum[/i] infection in neonatal mice - Archive ouverte HAL
Article Dans Une Revue PLoS Pathogens Année : 2013

Intestinal CD103+ dendritic cells are key players in the innate immune control of [i]Cryptosporidium parvum[/i] infection in neonatal mice

Louis Lantier
Sonia Lacroix-Lamandé
Yves Le Vern
Fabrice Laurent

Résumé

Cryptosporidium parvum is a zoonotic protozoan parasite found worldwide, that develops only in the gastrointestinal epithelium and causes profuse diarrhea. Using a mouse model of C. parvum infection, we demonstrated by conditional depletion of CD11c+ cells that these cells are essential for the control of the infection both in neonates and adults. Neonates are highly susceptible to C. parvum but the infection is self-limited, whereas adults are resistant unless immunocompromised. We investigated the contribution of DC to the age-dependent susceptibility to infection. We found that neonates presented a marked deficit in intestinal CD103+ DC during the first weeks of life, before weaning, due to weak production of chemokines by neonatal intestinal epithelial cells (IEC). Increasing the number of intestinal CD103+ DC in neonates by administering FLT3-L significantly reduced susceptibility to the infection. During infections in neonates, the clearance of the parasite was preceded by a rapid recruitment of CD103+ DC mediated by CXCR3-binding chemokines produced by IEC in response to IFN gamma. In addition to this key role in CD103+ DC recruitment, IFN gamma is known to inhibit intracellular parasite development. We demonstrated that during neonatal infection CD103+ DC produce IL-12 and IFN gamma in the lamina propria and the draining lymph nodes. Thus, CD103+ DC are key players in the innate immune control of C. parvum infection in the intestinal epithelium. The relative paucity of CD103+ DC in the neonatal intestine contributes to the high susceptibility to intestinal infection.
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hal-01204445 , version 1 (07-11-2016)

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Louis Lantier, Sonia Lacroix-Lamandé, Laurent Potiron, Coralie Metton, Françoise Drouet, et al.. Intestinal CD103+ dendritic cells are key players in the innate immune control of [i]Cryptosporidium parvum[/i] infection in neonatal mice. PLoS Pathogens, 2013, 9 (12), 16 p. ⟨10.1371/journal.ppat.1003801⟩. ⟨hal-01204445⟩
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