Communication Dans Un Congrès Journal of Clinical Gastroenterology Année : 2014

Protective effects of lactococci strains delivering either IL-10 protein or cDNA in a TNBS-induced chronic colitis model

Résumé

Background: Oral treatment with Lactococcus lactis strains secreting the anti-inflammatory cytokine interleukin (IL)-10 has previously shown success as a therapy for inflammatory bowel diseases (IBD). Goals: Our aim was to compare the protective effects of IL-10, delivered by recombinant lactoccoci using 2 novel expression systems, in a murine colitis model mimicking the relapsing nature of IBD. The first system is based on a Stress-Inducible Controlled Expression system for the production and delivery of heterologous proteins at mucosal surfaces and the second allows the delivery to the host cells of an il-10 cDNA cassette, harbored in a eukaryotic DNA expression vector (pValac). Study: Colitis was induced in female BALB/c mice by intrarectal injection of 2,4,6-trinitrobenzenesulphonic acid (TNBS). Mice that recovered received one of the bacteria treatments or saline solution orally during 14 days. Colitis was reactivated 25 days after the first TNBS injection with a second TNBS challenge. Three days after colitis reactivation, cytokine profiles and inflammation in colon samples were evaluated. Results: Animals (N=9) receiving L. lactis strains secreting IL-10 using Stress-Inducible Controlled Expression system or delivering pValac: il-10 plasmid showed lower weight loss (P < 0.005), lower damage scores (P < 0.005), and immune activation in their large intestines compared with inflamed nontreated mice. Conclusions: Our results confirm the protective effect of IL-10 delivered either as a protein or as a cDNA in a colitis model mimicking the relapsing nature of IBD and provides a step further in the "proof-of-concept" of genetically engineered bacteria as a valid system to deliver therapeutic molecules at mucosal level.

Dates et versions

hal-01204425 , version 1 (23-09-2015)

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Citer

Silvina del Carmen, Rebeca Martin Rosique, Tessalia Saraiva, Meritxell Zurita-Turk, Anderson Miyoshi, et al.. Protective effects of lactococci strains delivering either IL-10 protein or cDNA in a TNBS-induced chronic colitis model. 7. Probiotics, Prebiotics & New Foods Meeting, International Probiotics Association (IPA). ITA., Sep 2013, Rome, Italy. ⟨10.1097/MCG.0000000000000235⟩. ⟨hal-01204425⟩
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