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Article Dans Une Revue International Archives of Allergy and Immunology Année : 2009

Allergy therapy by intranasal administration with recombinant Lactococcus lactis Producing bovine beta-lactoglobulin

Résumé

In the last years, the use of probiotics such as lactic acid bacteria (LAB) has been proposed as an attractive alternative for the management of allergic diseases. A partial prevention from sensitization to bovine β-lactoglobulin (BLG), one of the major cows’ milk allergens, could be achieved in mice after intranasal administration with a recombinant LAB strain, Lactococcus lactis, producing BLG (LL-BLG). This study aimed to evaluate the effects of the LL-BLG strain in a therapeutic protocol. Methods: Three groups of mice were first orally sensitized to cows’ milk and then intranasally administered with either the LL-BLG strain, BLG protein alone or saline solution. Serum samples were collected to analyze BLG-specific IgE, IgG1 and IgG2a, and mice were further intranasally challenged with BLG to elicit a specific allergic reaction. Results: Treatment with LL-BLG, but not with BLG alone, contributed to diminish IgG1 production in serum and bronchoalveolar lavage fluids. This was associated with decreased IL-4 production and enhanced IFN-γ production by BLG-reactivated splenocytes, suggesting a switch from Th2- to Th1-immune response. Furthermore, we observed that administration of LL-BLG or LL locally reduced the allergic reaction induced after intranasal challenge, as evidenced by decreased release of IL-4 in bronchoalveolar lavage fluids. Conclusion: These preliminary results demonstrate the efficiency of the intranasal administration of LL-BLG for specific therapy against cows’ milk-related allergy.
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Dates et versions

hal-01204327 , version 1 (23-09-2015)

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Naima Bermudez, Sandrine Ah-Leung-Poullias, Luis Bermudez Humaran, Gerard Corthier, Philippe Langella, et al.. Allergy therapy by intranasal administration with recombinant Lactococcus lactis Producing bovine beta-lactoglobulin. International Archives of Allergy and Immunology, 2009, 150 (1), pp.25-31. ⟨10.1159/000210377⟩. ⟨hal-01204327⟩
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