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Article Dans Une Revue Plant Physiology Année : 2014

Catalytic subunit stoichiometry within the cellulose synthase complex

Résumé

Cellulose synthesis is driven by large plasma membrane-inserted protein complexes, which in plants have 6-fold symmetry. In Arabidopsis (Arabidopsis thaliana), functional cellulose synthesis complexes (CSCs) are composed of at least three different cellulose synthase catalytic subunits (CESAs), but the actual ratio of the CESA isoforms within the CSCs remains unresolved. In this work, the stoichiometry of the CESAs in the primary cell wall CSC was determined, after elimination of CESA redundancy in a mutant background, by coimmunoprecipitation and mass spectrometry using label-free quantitative methods. Based on spectral counting, we show that CESA1, CESA3, and CESA6 are present in a 1:1:1 molecular ratio.

Dates et versions

hal-01204130 , version 1 (23-09-2015)

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Martine Gonneau, Thierry Desprez, Alain Guillot, Samantha Vernhettes, Herman Höfte. Catalytic subunit stoichiometry within the cellulose synthase complex. Plant Physiology, 2014, 166 (4), pp.1709-1712. ⟨10.1104/pp.114.250159⟩. ⟨hal-01204130⟩
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