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Communication Dans Un Congrès Année : 2013

Cartography of tomato pericarp cellular morphology at the fruit scale

Résumé

Consumer perception and industrial processing of fleshy fruits greatly depend on the morphology and spatial organization of cells within the tissues. To study the relations between the structure and the mechanical properties of fleshy fruits, it is necessary to quantify their cellular morphology. A generic approach is proposed to build cartographies of cellular morphology at the fruit scale, depicting regions corresponding to different cell morphologies. The approach is based on sampling the whole fruit at known positions, imaging and quantifying the local cellular morphology, pooling measurements to take into account the biological variability and projecting results in a 3D model of the whole fruit. The result is a synthetic representation of cell morphology variations within the whole fruit. Tomato pericarp was used as a model for fleshy fruits. Two different scales were investigated through 3D imaging using confocal microscopy, and macroscopy imaging of pericarp slices. The morphology of the tomato pericarp was assessed (1) from 3D microscopy images through stereological estimation of cell wall surface area and (2) from greyscale granulometry computed with mathematical morphology. Spatial heterogeneity within pericarp was described by measuring the morphology for different regions of interest within the whole fruit and for each characterisation method. This resulted in cartography of the cellular structure at the scale of the fruit; either by cell wall surface area estimate or by grey level mean size. Both methods presented complementary results, and made it possible to compare results obtained at different scales.

Domaines

Chimie organique
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Dates et versions

hal-01204126 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-01204126 , version 1
  • PRODINRA : 283595

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David Legland, Marie Francoise Devaux, Brigitte Bouchet, Fabienne Guillon, Marc Lahaye. Cartography of tomato pericarp cellular morphology at the fruit scale. Insidefood Symposium 2013, Apr 2013, Leuven, Belgium. ⟨hal-01204126⟩
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