A correlative microscopy approach relates microtubule behaviour, local organ geometry, and cell growth at the Arabidopsis shoot apical meristem - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Botany Année : 2013

A correlative microscopy approach relates microtubule behaviour, local organ geometry, and cell growth at the Arabidopsis shoot apical meristem

Résumé

Cortical microtubules (CMTs) are often aligned in a particular direction in individual cells or even in groups of cells and play a central role in the definition of growth anisotropy. How the CMTs themselves are aligned is not well known, but two hypotheses have been proposed. According to the first hypothesis, CMTs align perpendicular to the maximal growth direction, and, according to the second, CMTs align parallel to the maximal stress direction. Since both hypotheses were formulated on the basis of mainly qualitative assessments, the link between CMT organization, organ geometry, and cell growth is revisited using a quantitative approach. For this purpose, CMT orientation, local curvature, and growth parameters for each cell were measured in the growing shoot apical meristem (SAM) of Arabidopsis thaliana. Using this approach, it has been shown that stable CMTs tend to be perpendicular to the direction of maximal growth in cells at the SAM periphery, but parallel in the cells at the boundary domain. When examining the local curvature of the SAM surface, no strict correlation between curvature and CMT arrangement was found, which implies that SAM geometry, and presumed geometry-derived stress distribution, is not sufficient to prescribe the CMT orientation. However, a better match between stress and CMTs was found when mechanical stress derived from differential growth was also considered.
Fichier principal
Vignette du fichier
burian_2013_1.pdf (6.61 Mo) Télécharger le fichier
burian_2013_sequence_video_2.avi (18 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01204036 , version 1 (28-05-2020)

Identifiants

Citer

Agata Burian, Michal Ludynia, Magalie Uyttewaal, Jan Traas, Arezki Boudaoud, et al.. A correlative microscopy approach relates microtubule behaviour, local organ geometry, and cell growth at the Arabidopsis shoot apical meristem. Journal of Experimental Botany, 2013, 64 (18), pp.5753-5767. ⟨10.1093/jxb/ert352⟩. ⟨hal-01204036⟩
116 Consultations
21 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More