Warm temperatures induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in [i]Arabidopsis[/i] - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2013

Warm temperatures induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in [i]Arabidopsis[/i]

Abstract

Owing to their sessile nature, plants have evolved sophisticated genetic and epigenetic regulatory systems to respond quickly and reversibly to daily and seasonal temperature changes. However, our knowledge of how plants sense and respond to warming ambient temperatures is rather limited. Here we show that an increase in growth temperature from 22 degrees C to 30 degrees C effectively inhibited transgene-induced posttranscriptional gene silencing (PTGS) in Arabidopsis. Interestingly, warmth-induced PTGS release exhibited transgenerational epigenetic inheritance. We discovered that the warmth-induced PTGS release occurred during a critical step that leads to the formation of double-stranded RNA (dsRNA) for producing small interfering RNAs (siRNAs). Deep sequencing of small RNAs and RNA blot analysis indicated that the 22-30 degrees C increase resulted in a significant reduction in the abundance of many trans-acting siRNAs that require dsRNA for biogenesis. We discovered that the temperature increase reduced the protein abundance of SUPPRESSOR OF GENE SILENCING 3, as a consequence, attenuating the formation of stable dsRNAs required for siRNA biogenesis. Importantly, SUPPRESSOR OF GENE SILENCING 3 overexpression released the warmth-triggered inhibition of siRNA biogenesis and reduced the transgenerational epigenetic memory. Thus, our study reveals a previously undescribed association between warming temperatures, an epigenetic system, and siRNA biogenesis.
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hal-01203983 , version 1 (29-05-2020)

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Si-Hui Zhong, Jun-Zhong Liu, Hua Jin, Lin Lin, Qun Li, et al.. Warm temperatures induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in [i]Arabidopsis[/i]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (22), pp.9171-9176. ⟨10.1073/pnas.1219655110⟩. ⟨hal-01203983⟩
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