Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation
Résumé
Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental
pulp stem cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic
differentiation of DPSCs. Integrated Raman intensities in the 2800 to 3000 cm−1 region (C-H stretching) and the
960cm−1 peak (ν1) were collected (to image cells and phosphate, respectively), and the ratio of two peaks
1660 over 1690 cm−1 (amide I bands) to measure the collagen cross-linking has been calculated. Raman spectra of DPSCs after 21 days differentiation reveal several phosphate peaks: ν1 (first stretching mode) at 960 cm−1, ν2 at 430 cm−1, and ν4 at 585 cm−1 and collagen cross-linking can also be calculated. Confocal Raman microscopy enables monitoring osteogenic differentiation in vitro and can be a credible tool for clinical stem cell based research.