Toward an Embedded System For The In-Vitro and In-Situ Cell Proliferation Characterization by Impedance Spectroscopy
Résumé
Wireless Implantable systems are of primary
interest for the in situ chronic monitoring or regulation of
physiological parameters. Nevertheless, the implantation of such
devices leads to immune system responses including an abnormal
cell proliferation in its immediate surroundings. Both functioning
and performances can be altered. So, it is necessary to monitor
the evolution of the fibrotic tissues to prevent the dysfunctions.
ECIS technique is appropriate for that. In this article, we
propose, as a first step, an embedded system based on this
technique for continuous bio-impedance measurements and
designed for an arrangement in a culture chamber. It features a
wide frequency range analysis with a view to determining an
electrical marker of the cell type as a function of the frequency
response. A validation of the system, for measurements on
human fibroblasts, shows a very good match with the usual Cole-
Cole model with an average error of only 1.5% on a 8-days
experiment performed in real conditions.