Identification of DNA Motifs Implicated in Maintenance of Bacterial Core Genomes by Predictive Modeling - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS Genetics Année : 2007

Identification of DNA Motifs Implicated in Maintenance of Bacterial Core Genomes by Predictive Modeling

Résumé

Bacterial biodiversity at the species level, in terms of gene acquisition or loss, is so immense that it raises the question of how essential chromosomal regions are spared from uncontrolled rearrangements. Protection of the genome likely depends on specific DNA motifs that impose limits on the regions that undergo recombination. Although most such motifs remain unidentified, they are theoretically predictable based on their genomic distribution properties. We examined the distribution of the ‘‘crossover hotspot instigator,’’ or Chi, in Escherichia coli, and found that its exceptional distribution is restricted to the core genome common to three strains. We then formulated a set of criteria that were incorporated in a statistical model to search core genomes for motifs potentially involved in genome stability in other species. Our strategy led us to identify and biologically validate two distinct heptamers that possess Chi properties, one in Staphylococcus aureus, and the other in several streptococci. This strategy paves the way for widescale discovery of other important functional noncoding motifs that distinguish core genomes from the strain-variable regions.

Domaines

Génétique
Fichier principal
Vignette du fichier
49959_20120510094742452_1.pdf (283.74 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01197542 , version 1 (31-05-2020)

Identifiants

Citer

David Halpern, Helene Chiapello, Sophie Schbath, Stephane Robin, Christelle Hennequet-Antier, et al.. Identification of DNA Motifs Implicated in Maintenance of Bacterial Core Genomes by Predictive Modeling. PLoS Genetics, 2007, 3 (9), pp.1614-1621;e153. ⟨10.1371/journal.pgen.0030153⟩. ⟨hal-01197542⟩
113 Consultations
44 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More