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                <title level="m">Rencontres autour des Recherches sur les  Ruminants</title>
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              <p>In cattle, peri-implantation is a critical step associated to the highest rate of pregnancy failures. Our project aims at identifying endometrial factors that play crucial functions at implantation. Using a bovine microarray (13330 oligonucleotides) dedicated to the study of pregnancy, expression profiles of the endometrium were established at day 20 of the oestrous cycle or pregnancy. Pregnancy deeply affects various biological functions in the endometrium including “cell growth and differentiation”, “immune response” or “metabolism”. Twenty genes were selected and their differential expressions were confirmed by quantitative RT-PCR, their cellular localisations were analysed by in situ hybridisation, and the direct regulation by IFNtau was established using primary cultures of bovine endometrial cells. Our data may contribute to identify new genes as potential biological markers for early pregnancy.</p>
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              <p>Chez les bovins, la période péri-implantatoire est une étape critique, associée à l’essentiel des échecs de gestation. L’objectif principal de ce projet vise à identifier des facteurs endométriaux cruciaux lors de l’implantation. A partir d’endomètres bovins (vingt jours de cycle ou de gestation) et de puces bovines de 13300 oligonucléotides, les profils d’expression génique montrent que la gestation affecte le transcriptome endométrial avec un impact majeur sur la croissance et la différenciation cellulaire, la réponse immunitaire ou le métabolisme. Les niveaux d’expression d’une sélection de gènes ont été confirmés par qRT-PCR, leurs localisations cellulaires analysées par hybridation in situ. La régulation directe par l’IFNtau des gènes sélectionnés a été établie à l’aide de cultures primaires endométriales. Nos données peuvent contribuer à identifier de nouveaux facteurs présentant un intérêt potentiel comme marqueurs de la gestation précoce.</p>
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