Investigation of [i]Geotrichum candidum[/i] gene expression during the ripening of Reblochon-type cheese by reverse transcription-quantitative PCR - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Food Microbiology Année : 2015

Investigation of [i]Geotrichum candidum[/i] gene expression during the ripening of Reblochon-type cheese by reverse transcription-quantitative PCR

Résumé

Cheese ripening involves the activity of various bacteria, yeasts or molds, which contribute to the development of the typical color, flavor and texture of the final product. In situ measurements of gene expression are increasingly being used to improve our understanding of the microbial flora activity in cheeses. The objective of the present study was to investigate the physiology and metabolic activity of Geotrichum candidum during the ripening of Reblochon-type cheeses by quantifying mRNA transcripts at various ripening times. The expression of 80 genes involved in various functions could be quantified with a correct level of biological repeatability using a set of three stable reference genes. As ripening progresses, a decrease in expression was observed for genes involved in cell wall organization, translation, vesicular mediated transport, and in cytoskeleton constituents and ribosomal protein genes. There was also a decrease in the expression of mitochondrial F1F0 ATP synthase and plasma membrane H+ ATPase genes. Some genes involved in the catabolism of lactate, acetate and ethanol were expressed to a greater extent at the beginning of ripening. During the second part of ripening, there was an increased expression of genes involved in the transport and catabolism of amino acids, which could be attributed to a change in the energy source. There was also an increase in the expression of genes involved in autophagy and of genes possibly involved in lifespan determination. Quantification of mRNA transcripts may also be used to produce bioindicators relevant for cheesemaking, for example when considering genes encoding enzymes involved in the catabolism of amino acids.
Fichier non déposé

Dates et versions

hal-01195507 , version 1 (07-09-2015)

Identifiants

Citer

Jessie Castellote, Sebastien Fraud, Francoise Irlinger, Dominique Swennen, Frédéric Fer, et al.. Investigation of [i]Geotrichum candidum[/i] gene expression during the ripening of Reblochon-type cheese by reverse transcription-quantitative PCR. International Journal of Food Microbiology, 2015, 194, pp.54-61. ⟨10.1016/j.ijfoodmicro.2014.11.009⟩. ⟨hal-01195507⟩
77 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More