High-throughput sequencing analyses of XX genital ridges lacking [i]FOXL2[/i] reveal [i]DMRT1[/i] up-regulation before [i]SOX9[/i] expression during the sex-reversal process in goats - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of Reproduction Année : 2014

High-throughput sequencing analyses of XX genital ridges lacking [i]FOXL2[/i] reveal [i]DMRT1[/i] up-regulation before [i]SOX9[/i] expression during the sex-reversal process in goats

Luc Jouneau
Eric Pailhoux

Résumé

FOXL2 loss of function in goats leads to the early transdifferentiation of ovaries into testes, then to the full sex reversal of XX homozygous mutants. By contrast, Foxl2 loss of function in mice induces an arrest of follicle formation after birth, followed by complete female sterility. In order to understand the molecular role of FOXL2 during ovarian differentiation in the goat species, putative FOXL2 target genes were determined at the earliest stage of gonadal sex-specific differentiation by comparing the mRNA profiles of XX gonads expressing the FOXL2 protein or not. Of these 163 deregulated genes, around two-thirds corresponded to testicular genes that were up-regulated when FOXL2 was absent, and only 19 represented female-associated genes, down-regulated in the absence of FOXL2. FOXL2 should therefore be viewed as an antitestis gene rather than as a female-promoting gene. In particular, the key testis-determining gene DMRT1 was found to be up-regulated ahead of SOX9, thus suggesting in goats that SOX9 primary up-regulation may require DMRT1. Overall, our results equated to FOXL2 being an antitestis gene, allowing us to propose an alternative model for the sex-determination process in goats that differs slightly from that demonstrated in mice.

Dates et versions

hal-01194038 , version 1 (04-09-2015)

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Maeva El Zaiat, Luc Jouneau, Dominique Thepot Thépot, Christophe C. Klopp, Aurélie Allais-Bonnet, et al.. High-throughput sequencing analyses of XX genital ridges lacking [i]FOXL2[/i] reveal [i]DMRT1[/i] up-regulation before [i]SOX9[/i] expression during the sex-reversal process in goats. Biology of Reproduction, 2014, 91 (6), pp.1-14. ⟨10.1095/biolreprod.114.122796⟩. ⟨hal-01194038⟩
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