Enzyme collagen membrane for electrochemical determination of glucose - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 1979

Enzyme collagen membrane for electrochemical determination of glucose

Résumé

A new trace glucose analyzer has been designed using electrochemical sensors. The differential device includes (a) a glucose sensor consisting of a modified gas electrode in which ihe pH detector was replaced by a platinum disk and the porous film by a collagen membrane on which beta-D-glucose oxidase has been covalently bound after an acyl-azide activation process; (b) a compensating electrode mounted with a nonenzymatic collagen membrane. After injection of a glucose containing sample into the reaction vessel, where the probes are immersed, an anodic current is detected at the enzyme working electrode. Current outputs of both electrodes are subtracted and twice differentiated; a steady state is reached and the stationary and dynamic responses are recorded. Both responses are proportional to glucose concentration in the 0.1 µM-2 mM range, and the reproducibility was found to be better than 2% using these conditions. The extreme sensitivity exhibited by our system, i.e., 10 nM, is better than previously reported data by 3 orders of magnitude, and is very favorable for trace glucose assays in food and biological samples.
Fichier principal
Vignette du fichier
ENPC00_AR_LEESU_0009.pdf (17.53 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01179308 , version 1 (23-07-2015)

Identifiants

Citer

Daniel R. Thevenot, Robert Sternberg, Pierre R. Coulet, Jean Laurent, Danièle C. Gautheron. Enzyme collagen membrane for electrochemical determination of glucose. Analytical Chemistry, 1979, 51, pp.96 - 100. ⟨10.1021/ac50037a031⟩. ⟨hal-01179308⟩
168 Consultations
178 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More