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Communication Dans Un Congrès Année : 2014

Systems biology of piglet maturity with focus on muscle metabolism

Résumé

In recent decades, there has been improvement of sow prolificacy and body composition but the genetic progress has been accompanied by a substantial increase in piglet mortality before weaning. The most critical period is the first 48 hours after birth. The maturity of piglets, defined as the state of full development for survival at birth, is an important determinant of early mortality. The objective of our project is to take advantage of current knowledge about two pig breeds, Large White piglets with increased mortality and Meishan piglets being more robust at birth. Maturity of several tissues and metabolite profiles of various fluids were analyzed on the fetuses (LW, MS and reciprocal F1) at day 90 and 110 of gestation (birth at day 114). As example, the results of muscle tissue obtained by an integrative approach (with transcriptomic, proteomic and physiological data) will be presented. Muscle tissue plays a key role at birth, not only to provide immediate locomotion particularly to reach the teats, but also because it is involved in thermoregulation through mobilization of energy reserves (especially glycogen). In this context, we identified 5,739 genes (annotated and unique) and 241 differential protein spots using a linear mixed model (Bonferroni adjusted p-value 1%) from which 1,120 genes and 27 proteins are influenced by the interaction between the genotype and the gestational stage. The analysis of the interaction highlighted genes and functions involved in the different neonatal maturity observed between Large White and Meishan. In particular, key genes for gluconeogenesis/glycolysis were over-expressed in Meishan. Furthermore, the analysis revealed that 367 genes were differentially expressed between the reciprocal crossbred fetuses and most of them (288 genes) exhibited a paternal preferential expression. Moreover, we expect to identify biomarkers related to the determinism of the maturity in order to develop a strategy in quantitative genetics.
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Dates et versions

hal-01173403 , version 1 (07-07-2015)

Identifiants

  • HAL Id : hal-01173403 , version 1
  • PRODINRA : 276980

Citer

Valentin Voillet, Louis Lefaucheur, Laurianne L. Canario, Marie-Christine Pere, Alain Paris, et al.. Systems biology of piglet maturity with focus on muscle metabolism. 34. International Society for Animal Genetics Conference (ISAG), Jul 2014, Xi'an, China. ⟨hal-01173403⟩
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