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Journal Articles Scientific Reports Year : 2015

Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR

Abstract

We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in beta-alanine signals in the thorax of flies showing muscle degeneration.

Dates and versions

hal-01171918 , version 1 (06-07-2015)

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Vincent Sarou-Kanian, Nicolas Joudiou, Fanny Louat, Maxime Yon, Frédéric Szeremeta, et al.. Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR. Scientific Reports, 2015, 5 (9872), doi:10.1038/srep09872. ⟨10.1038/srep09872⟩. ⟨hal-01171918⟩
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