Architecture dependence of actin filament network disassembly - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Current Biology - CB Année : 2015

Architecture dependence of actin filament network disassembly

Résumé

Turnover of actin networks in cells requires the fast disassembly of aging actin structures. While ADF/cofilin and Aip1 have been identified as central players, how their activities are modulated by the architecture of the networks remains unknown. Using our ability to reconstitute a diverse array of cellular actin organizations, we found that ADF/cofilin binding and ADF/cofilin-mediated disassembly both depend on actin geometrical organization. ADF/cofilin decorates strongly and stabilizes actin cables, whereas its weaker interaction to Arp2/3 complex networks is correlated with their dismantling and their reorganization into stable architectures. Cooperation of ADF/cofilin with Aip1 is necessary to trigger the full disassembly of all actin filament networks. Additional experiments performed at the single-molecule level indicate that this cooperation is optimal above a threshold of 23 molecules of ADF/cofilin bound as clusters along an actin filament. Our results indicate that although ADF/cofilin is able to dismantle selectively branched networks through severing and debranching, stochastic disassembly of actin filaments by ADF/cofilin and Aip1 represents an efficient alternative pathway for the full disassembly of all actin networks. Our data support a model in which the binding of ADF/cofilin is required to trigger a structural change of the actin filaments, as a prerequisite for their disassembly by Aip1.
Fichier principal
Vignette du fichier
GGBM.pdf (1.61 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01166758 , version 1 (26-09-2017)

Identifiants

Citer

Laurène Gressin, Audrey Guillotin, Christophe Guérin, Laurent Blanchoin, Alphée Michelot. Architecture dependence of actin filament network disassembly. Current Biology - CB, 2015, 25 (11), pp.1437-1447. ⟨10.1016/j.cub.2015.04.011⟩. ⟨hal-01166758⟩
81 Consultations
73 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More