Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Diseases of Aquatic Organisms Année : 2003

Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis.

Résumé

Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V. tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V. tapetis compared to that of V. splendidus and V. pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
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hal-01154283 , version 1 (19-12-2021)

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  • HAL Id : hal-01154283 , version 1
  • PUBMED : 14735928

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Gwénaëlle Choquet, Philippe Soudant, Christophe Lambert, Jean-Louis Nicolas, Christine Paillard. Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis.. Diseases of Aquatic Organisms, 2003, 57 (1-2), pp.109-16. ⟨hal-01154283⟩
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