In situ application of non-thermal plasma: preliminary investigations for colorectal and lung tolerance
Résumé
Non-thermal plasma (NTP) has a significant antitumor activity in vitro
on different cell lines including brain, colorectal and lung tumor. The necessary
dose to kill 50% of cells was ~9J/cm² for these different cell lines. Reactive
Oxygen Species (ROS) generated during treatment appears to be responsible of
this effect and induce DNA damages together with a cell cycle arrest leading to
an apoptosis induction. These promising results in vitro lead us to evaluate NTP
in vivo. The tolerance study was conducted with NF-kB-luc reported mice. NFkB
is a transcription factor implied in inflammatory process and bioluminescence
imaging of this strain of mice allows monitoring of inflammation intensity and
time course. Tracheal intubation with Plasma Gun using a small catheter and low
gas flow was successfully achieved. As a preliminary result, an induction of the
inflammatory process was observed in the treated lung after 6 and 12 min of
treatment. Concerning colorectal inflammation following FE-DBD treatment,
NF-kB mice failed to reveal any inflammation in preliminary experiments.
Further investigations are needed to elaborate a treatment protocol for lung and
colorectal tumor treatment.
Keywords: Non-Thermal Plasma, Antitumor activity, Tolerance study