In situ application of non-thermal plasma: preliminary investigations for colorectal and lung tolerance - Archive ouverte HAL
Communication Dans Un Congrès Année : 2011

In situ application of non-thermal plasma: preliminary investigations for colorectal and lung tolerance

Résumé

Non-thermal plasma (NTP) has a significant antitumor activity in vitro on different cell lines including brain, colorectal and lung tumor. The necessary dose to kill 50% of cells was ~9J/cm² for these different cell lines. Reactive Oxygen Species (ROS) generated during treatment appears to be responsible of this effect and induce DNA damages together with a cell cycle arrest leading to an apoptosis induction. These promising results in vitro lead us to evaluate NTP in vivo. The tolerance study was conducted with NF-kB-luc reported mice. NFkB is a transcription factor implied in inflammatory process and bioluminescence imaging of this strain of mice allows monitoring of inflammation intensity and time course. Tracheal intubation with Plasma Gun using a small catheter and low gas flow was successfully achieved. As a preliminary result, an induction of the inflammatory process was observed in the treated lung after 6 and 12 min of treatment. Concerning colorectal inflammation following FE-DBD treatment, NF-kB mice failed to reveal any inflammation in preliminary experiments. Further investigations are needed to elaborate a treatment protocol for lung and colorectal tumor treatment. Keywords: Non-Thermal Plasma, Antitumor activity, Tolerance study
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Dates et versions

hal-01149342 , version 1 (06-05-2015)

Identifiants

  • HAL Id : hal-01149342 , version 1

Citer

Marc Vandamme, Eric Robert, Julien Sobilo, Vanessa Sarron, Delphine Ries, et al.. In situ application of non-thermal plasma: preliminary investigations for colorectal and lung tolerance. ISPC 20, IPCS, Jul 2011, Philadelphie, United States. ⟨hal-01149342⟩
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