Overexpression of StDREB1 Transcription Factor Increases Tolerance to Salt in Transgenic Potato Plants
Résumé
It has been established that drought-responsive
element binding (DREB) proteins correspond to transcription
factors which play important regulatory roles in plant response to abiotic and biotic stresses. In this study, a novel cDNA encoding DREB transcription factor, designated
StDREB1, was isolated from potato (Solanum tuberosum L.). This protein was classified in the A-4 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Semi-quantitative
RT-PCR showed that StDREB1 is expressed in leaves,
stems, and roots under stress conditions and it is greatly
induced by NaCl, drought, low temperature, and abscisic
acid (ABA) treatments. Overexpression of StDREB1 cDNA
in transgenic potato plants exhibited an improved salt and
drought stress tolerance in comparison to the non-transformed controls. The enhanced stress tolerance may be
associated with the increase in P5CS-RNA expression (d1-
pyrroline-5-carboxylate synthetase) and the subsequent
accumulation of proline osmoprotectant in addition to a
better control of water loss. Overexpression of StDREB1
also activated stress-responsive genes, such as those
encoding calcium-dependent protein kinases (CDPKs), in
transgenic potatoes under standard and high salt conditions.
These data suggest that the StDREB1 transcription factor is
involved in the regulation of salt stress tolerance in potato by the activation of different downstream gene expression.
Domaines
Génétique des plantes
Origine : Fichiers produits par l'(les) auteur(s)
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