Effects of Partial 02 Pressure, Partial CO2 Pressure, and Agitation on Growth Kinetics of Azospirillum lipoferum under Fermentor Conditions - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 1990

Effects of Partial 02 Pressure, Partial CO2 Pressure, and Agitation on Growth Kinetics of Azospirillum lipoferum under Fermentor Conditions

Résumé

Azospirillum lipoferum crtl was grown in batch cultures under standard conditions at 85% saturation of dissolved oxygen (DO) and 30-g/liter glucose concentrations. Kinetic studies revealed nutritional limitations of growth and the presence of an initial lag phase prior to consumption of glucose. The influences of various gaseous environments and shear stress on growth, i.e., various conditions of agitation-aeration, were characterized. Faster growth in the first stages of the culture and shorter duration of the lag phase were observed at DO concentrations of <30% saturation. The possible influences of dissolved CO2 concentration or shear stress or both were discounted, and we confirmed the detrimental effect of high DO levels (up to 80% saturation) and the favorable influence of low DO concentrations (lower than 30% saturation) on growth. It was concluded that the gaseous environment, i.e., the DO concentration, needs to be considered as an operating parameter and be accurately controlled to ensure optimal growth of Azospirillum cells.
Fichier principal
Vignette du fichier
1990-Paul-AEM.pdf (863.18 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01142178 , version 1 (14-04-2015)

Identifiants

  • HAL Id : hal-01142178 , version 1

Citer

Etienne Paul, Daniel Mulard, Philippe Blanc, Jacques Fages, Gerard Goma, et al.. Effects of Partial 02 Pressure, Partial CO2 Pressure, and Agitation on Growth Kinetics of Azospirillum lipoferum under Fermentor Conditions. Applied and Environmental Microbiology, 1990, 56 (11), pp.3235-3239. ⟨hal-01142178⟩
113 Consultations
78 Téléchargements

Partager

Gmail Facebook X LinkedIn More