Functional expression of plant membrane proteins in Lactococcus lactis. - Archive ouverte HAL Access content directly
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Functional expression of plant membrane proteins in Lactococcus lactis.

Abstract

The study of most membrane proteins remains challenging due to their hydrophobicity and their low natural abundance in cells. Lactococcus lactis, a Gram-positive lactic bacterium, has been traditionally used in food fermentations and is nowadays widely used in biotechnology for large-scale production of heterologous proteins. This system has been successfully used for the production of prokaryotic and eukaryotic membrane proteins. The purpose of this chapter is to provide detailed protocols for (1) the expression of plant peripheral or intrinsic membrane proteins and then for (2) their solubilization, from Lactococcus membranes, for further purification steps and biochemical characterization.
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hal-01141092 , version 1 (10-04-2015)

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Sylvain Boutigny, Emeline Sautron, Annie Frelet-Barrand, Lucas Moyet, Daniel Salvi, et al.. Functional expression of plant membrane proteins in Lactococcus lactis.. Methods in molecular biology, 1258, Humana Press Inc., pp.147-65, 2015, Methods in Molecular Biology, 978-1-4939-2205-5 978-1-4939-2204-8. ⟨10.1007/978-1-4939-2205-5_8⟩. ⟨hal-01141092⟩
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