Site-specific cation release drives actin filament severing by vertebrate cofilin. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2014

Site-specific cation release drives actin filament severing by vertebrate cofilin.

Résumé

Actin polymerization powers the directed motility of eukaryotic cells. Sustained motility requires rapid filament turnover and subunit recycling. The essential regulatory protein cofilin accelerates network remodeling by severing actin filaments and increasing the concentration of ends available for elongation and subunit exchange. Although cofilin effects on actin filament assembly dynamics have been extensively studied, the molecular mechanism of cofilin-induced filament severing is not understood. Here we demonstrate that actin filament severing by vertebrate cofilin is driven by the linked dissociation of a single cation that controls filament structure and mechanical properties. Vertebrate cofilin only weakly severs Saccharomyces cerevisiae actin filaments lacking this "stiffness cation" unless a stiffness cation-binding site is engineered into the actin molecule. Moreover, vertebrate cofilin rescues the viability of a S. cerevisiae cofilin deletion mutant only when the stiffness cation site is simultaneously introduced into actin, demonstrating that filament severing is the essential function of cofilin in cells. This work reveals that site-specific interactions with cations serve a key regulatory function in actin filament fragmentation and dynamics.

Dates et versions

hal-01140866 , version 1 (09-04-2015)

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Hyeran Kang, Michael J Bradley, Wenxiang Cao, Kaifeng Zhou, Elena E Grintsevich, et al.. Site-specific cation release drives actin filament severing by vertebrate cofilin.. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (50), pp.17821-6. ⟨10.1073/pnas.1413397111⟩. ⟨hal-01140866⟩
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