<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-01131260</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-19T08:08:46+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">300-Fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization.</title>
            <author role="aut">
              <persName>
                <forename type="first">Colin J</forename>
                <surname>Jackson</surname>
              </persName>
              <idno type="halauthorid">898637-0</idno>
              <affiliation ref="#struct-1131"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christopher W</forename>
                <surname>Coppin</surname>
              </persName>
              <idno type="halauthorid">898638-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Paul D</forename>
                <surname>Carr</surname>
              </persName>
              <idno type="halauthorid">898639-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alexey</forename>
                <surname>Aleksandrov</surname>
              </persName>
              <email type="md5">db60c5cbda42b17098ffef5514efb285</email>
              <email type="domain">polytechnique.edu</email>
              <idno type="idhal" notation="string">alexey-aleksandrov</idno>
              <idno type="idhal" notation="numeric">740208</idno>
              <idno type="halauthorid" notation="string">21964-740208</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-8150-3931</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Matthew</forename>
                <surname>Wilding</surname>
              </persName>
              <idno type="halauthorid">898640-0</idno>
              <affiliation ref="#struct-18781"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Elena</forename>
                <surname>Sugrue</surname>
              </persName>
              <idno type="idhal" notation="numeric">773533</idno>
              <idno type="halauthorid" notation="string">898641-773533</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5014-9522</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Joanna</forename>
                <surname>Ubels</surname>
              </persName>
              <idno type="halauthorid">898642-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Michael</forename>
                <surname>Paks</surname>
              </persName>
              <idno type="halauthorid">898643-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Janet</forename>
                <surname>Newman</surname>
              </persName>
              <idno type="halauthorid">898644-0</idno>
              <affiliation ref="#struct-305635"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Thomas S</forename>
                <surname>Peat</surname>
              </persName>
              <idno type="halauthorid">898645-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Robyn J</forename>
                <surname>Russell</surname>
              </persName>
              <idno type="halauthorid">898646-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Martin J</forename>
                <surname>Field</surname>
              </persName>
              <email type="md5">18c80056b2d4cce41247cc83b4363425</email>
              <email type="domain">ibs.fr</email>
              <idno type="idhal" notation="numeric">968528</idno>
              <idno type="halauthorid" notation="string">927347-968528</idno>
              <orgName ref="#struct-576"/>
              <affiliation ref="#struct-576"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Martin</forename>
                <surname>Weik</surname>
              </persName>
              <idno type="halauthorid">184276-0</idno>
              <affiliation ref="#struct-576"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">John G</forename>
                <surname>Oakeshott</surname>
              </persName>
              <idno type="halauthorid">898647-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Colin</forename>
                <surname>Scott</surname>
              </persName>
              <idno type="halauthorid">898648-0</idno>
              <affiliation ref="#struct-255551"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Frank</forename>
                <surname>Thomas</surname>
              </persName>
              <email type="md5">d93af13e6932f3e24cc9a230ad329114</email>
              <email type="domain">ibs.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2015-03-13 11:14:35</date>
              <date type="whenModified">2025-09-27 19:20:06</date>
              <date type="whenReleased">2015-03-13 11:14:35</date>
              <date type="whenProduced">2014-07</date>
              <ref type="externalLink" target="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054219"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="139172">
                <persName>
                  <forename>Frank</forename>
                  <surname>Thomas</surname>
                </persName>
                <email type="md5">d93af13e6932f3e24cc9a230ad329114</email>
                <email type="domain">ibs.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-01131260</idno>
            <idno type="halUri">https://hal.science/hal-01131260</idno>
            <idno type="halBibtex">jackson:hal-01131260</idno>
            <idno type="halRefHtml">&lt;i&gt;Applied and Environmental Microbiology&lt;/i&gt;, 2014, 80 (13), pp.4003-11</idno>
            <idno type="halRef">Applied and Environmental Microbiology, 2014, 80 (13), pp.4003-11</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="UNIV-PARIS7" corresp="UNIV-PARIS">Université Denis Diderot - Paris VII</idno>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-GRENOBLE1">Université Joseph Fourier - Grenoble I</idno>
            <idno type="stamp" n="IJM">Institut Jacques Monod</idno>
            <idno type="stamp" n="IBS" corresp="CEA">Institut de Biologie Structurale</idno>
            <idno type="stamp" n="IBS-DYC" corresp="IBS">Groupe Dynamique et Cinétique des processus moléculaires / Dynamics and Kinetics of Molecular Processes Group (IBS-DYNAMOP)</idno>
            <idno type="stamp" n="CEA-DRF" corresp="CEA">Direction de Recherche Fondamentale</idno>
            <idno type="stamp" n="IRIG" corresp="CEA">Institut de Recherche Interdisciplinaire de Grenoble</idno>
            <idno type="stamp" n="CEA-GRE" corresp="CEA">CEA Grenoble</idno>
            <idno type="stamp" n="UNIV-PARIS">Université Paris Cité</idno>
            <idno type="stamp" n="UP-SCIENCES">Université Paris Cité - Faculté des Sciences</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">300-Fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization.</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Colin J</forename>
                    <surname>Jackson</surname>
                  </persName>
                  <idno type="halauthorid">898637-0</idno>
                  <affiliation ref="#struct-1131"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christopher W</forename>
                    <surname>Coppin</surname>
                  </persName>
                  <idno type="halauthorid">898638-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Paul D</forename>
                    <surname>Carr</surname>
                  </persName>
                  <idno type="halauthorid">898639-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alexey</forename>
                    <surname>Aleksandrov</surname>
                  </persName>
                  <email type="md5">db60c5cbda42b17098ffef5514efb285</email>
                  <email type="domain">polytechnique.edu</email>
                  <idno type="idhal" notation="string">alexey-aleksandrov</idno>
                  <idno type="idhal" notation="numeric">740208</idno>
                  <idno type="halauthorid" notation="string">21964-740208</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-8150-3931</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Matthew</forename>
                    <surname>Wilding</surname>
                  </persName>
                  <idno type="halauthorid">898640-0</idno>
                  <affiliation ref="#struct-18781"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Elena</forename>
                    <surname>Sugrue</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">773533</idno>
                  <idno type="halauthorid" notation="string">898641-773533</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5014-9522</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Joanna</forename>
                    <surname>Ubels</surname>
                  </persName>
                  <idno type="halauthorid">898642-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Michael</forename>
                    <surname>Paks</surname>
                  </persName>
                  <idno type="halauthorid">898643-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Janet</forename>
                    <surname>Newman</surname>
                  </persName>
                  <idno type="halauthorid">898644-0</idno>
                  <affiliation ref="#struct-305635"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Thomas S</forename>
                    <surname>Peat</surname>
                  </persName>
                  <idno type="halauthorid">898645-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Robyn J</forename>
                    <surname>Russell</surname>
                  </persName>
                  <idno type="halauthorid">898646-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Martin J</forename>
                    <surname>Field</surname>
                  </persName>
                  <email type="md5">18c80056b2d4cce41247cc83b4363425</email>
                  <email type="domain">ibs.fr</email>
                  <idno type="idhal" notation="numeric">968528</idno>
                  <idno type="halauthorid" notation="string">927347-968528</idno>
                  <orgName ref="#struct-576"/>
                  <affiliation ref="#struct-576"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Martin</forename>
                    <surname>Weik</surname>
                  </persName>
                  <idno type="halauthorid">184276-0</idno>
                  <affiliation ref="#struct-576"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">John G</forename>
                    <surname>Oakeshott</surname>
                  </persName>
                  <idno type="halauthorid">898647-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Colin</forename>
                    <surname>Scott</surname>
                  </persName>
                  <idno type="halauthorid">898648-0</idno>
                  <affiliation ref="#struct-255551"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">3064</idno>
                <idno type="issn">0099-2240</idno>
                <idno type="eissn">1098-5336</idno>
                <title level="j">Applied and Environmental Microbiology</title>
                <imprint>
                  <publisher>American Society for Microbiology</publisher>
                  <biblScope unit="volume">80</biblScope>
                  <biblScope unit="issue">13</biblScope>
                  <biblScope unit="pp">4003-11</biblScope>
                  <date type="datePub">2014-07</date>
                </imprint>
              </monogr>
              <idno type="pubmed">24771025</idno>
              <idno type="pubmedcentral">PMC4054219</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.bbm.bs">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Microbial metalloenzymes constitute a large library of biocatalysts, a number of which have already been shown to catalyze the breakdown of toxic chemicals or industrially relevant chemical transformations. However, while there is considerable interest in harnessing these catalysts for biotechnology, for many of the enzymes, their large-scale production in active, soluble form in recombinant systems is a significant barrier to their use. In this work, we demonstrate that as few as three mutations can result in a 300-fold increase in the expression of soluble TrzN, an enzyme from Arthrobacter aurescens with environmental applications that catalyzes the hydrolysis of triazine herbicides, in Escherichia coli. Using a combination of X-ray crystallography, kinetic analysis, and computational simulation, we show that the majority of the improvement in expression is due to stabilization of the apoenzyme rather than the metal ion-bound holoenzyme. This provides a structural and mechanistic explanation for the observation that many compensatory mutations can increase levels of soluble-protein production without increasing the stability of the final, active form of the enzyme. This study provides a molecular understanding of the importance of the stability of metal ion free states to the accumulation of soluble protein and shows that differences between apoenzyme and holoenzyme structures can result in mutations affecting the stability of either state differently.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-1131" status="OLD">
          <idno type="IdRef">026536412</idno>
          <idno type="RNSR">199712638E</idno>
          <orgName>Institut Jacques Monod</orgName>
          <orgName type="acronym">IJM (UMR_7592)</orgName>
          <date type="start">2009-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Université Paris Diderot, Bât. Buffon, 15 rue Hélène Brion, 75205 Paris cédex 13</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ijm.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR_7592" active="#struct-300301" type="direct"/>
            <relation name="UMR7592" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-18781" status="VALID">
          <orgName>Institute of Mathematical, Physics and Computer Sciences</orgName>
          <orgName type="acronym">IMAPCS</orgName>
          <desc>
            <address>
              <addrLine>Aberystwyth, SY23 3BZ</addrLine>
              <country key="GB"/>
            </address>
            <ref type="url">https://www.aber.ac.uk/en/impacs/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-321675" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-305635" status="INCOMING">
          <orgName>Universite de Californie</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="department" xml:id="struct-576" status="OLD">
          <idno type="IdRef">06912633X</idno>
          <idno type="ISNI">0000 0004 0641 5776</idno>
          <idno type="RNSR">199911764W</idno>
          <idno type="ROR">https://ror.org/04szabx38</idno>
          <orgName>Institut de biologie structurale</orgName>
          <orgName type="acronym">IBS - UMR 5075</orgName>
          <date type="start">1992-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Institut de Biologie Structurale, 71 avenue des martyrs, CS 10090, 38044 Grenoble Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ibs.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR5075 / UPR9015" active="#struct-441569" type="direct"/>
            <relation active="#struct-445543" type="direct"/>
            <relation name="DRF/IRIG/DBSC" active="#struct-555292" type="direct"/>
            <relation name="DRF/IRIG" active="#struct-465913" type="indirect"/>
            <relation active="#struct-300016" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-255551" status="INCOMING">
          <orgName>Udall Center for Studies in Public Policy</orgName>
          <desc>
            <address>
              <addrLine>School of Geography and Development University of Arizona, Tucson</addrLine>
              <country key="US"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-23624" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300301" status="OLD">
          <idno type="IdRef">027542084</idno>
          <idno type="ISNI">0000000121514068</idno>
          <idno type="ROR">https://ror.org/02n7qrg46</idno>
          <orgName>Université Paris Diderot - Paris 7</orgName>
          <orgName type="acronym">UPD7</orgName>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-paris-diderot.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-321675" status="VALID">
          <idno type="ROR">https://ror.org/015m2p889</idno>
          <orgName>Aberystwyth University</orgName>
          <desc>
            <address>
              <addrLine>Penglais, Aberystwyth, Ceredigion, SY23 3FL</addrLine>
              <country key="GB"/>
            </address>
            <ref type="url">https://www.aber.ac.uk/en/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-445543" status="OLD">
          <idno type="IdRef">188399275</idno>
          <idno type="ROR">https://ror.org/02rx3b187</idno>
          <orgName>Université Grenoble Alpes [2016-2019]</orgName>
          <orgName type="acronym">UGA [2016-2019]</orgName>
          <date type="start">2016-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>38058 Grenoble cedex</addrLine>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-555292" status="VALID">
          <idno type="IdRef">240648846</idno>
          <idno type="RNSR">201923169F</idno>
          <idno type="ROR">https://ror.org/00byxdz40</idno>
          <idno type="Wikidata">Q108737333</idno>
          <orgName>Institut de Recherche Interdisciplinaire de Grenoble</orgName>
          <orgName type="acronym">IRIG</orgName>
          <date type="start">2019-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://hal-cea.archives-ouvertes.fr/IRIG/</ref>
          </desc>
          <listRelation>
            <relation name="DRF/IRIG" active="#struct-465913" type="direct"/>
            <relation active="#struct-300016" type="indirect"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-465913" status="VALID">
          <idno type="IdRef">251118436</idno>
          <idno type="RNSR">201622496M</idno>
          <idno type="ROR">https://ror.org/01yvj5k91</idno>
          <idno type="Wikidata">Q52604525</idno>
          <orgName>Direction de Recherche Fondamentale (CEA)</orgName>
          <orgName type="acronym">DRF (CEA)</orgName>
          <date type="start">2016-01-01</date>
          <desc>
            <address>
              <addrLine>Direction de Recherche FondamentaleFundamental Research DivisionCEA Saclay91191 Gif-sur-Yvette</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://ceasciences.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-300016" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300016" status="VALID">
          <idno type="IdRef">026372061</idno>
          <idno type="ISNI">0000000122998025</idno>
          <idno type="ROR">https://ror.org/00jjx8s55</idno>
          <idno type="Wikidata">Q868550</idno>
          <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
          <orgName type="acronym">CEA</orgName>
          <desc>
            <address>
              <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cea.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-23624" status="VALID">
          <idno type="ROR">https://ror.org/03m2x1q45</idno>
          <orgName>University of Arizona</orgName>
          <desc>
            <address>
              <addrLine>Tucson AZ 85721 USA</addrLine>
              <country key="US"/>
            </address>
            <ref type="url">http://www.arizona.edu/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>