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Article Dans Une Revue Journal of Agricultural and Food Chemistry Année : 2011

Antimicrobial potential of egg Yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg

Résumé

Chicken egg ovoinhibitor is a multidomain Kazal-type serine protease inhibitor with unknown function. Comparison of expression between different tissues indicated that ovoinhibitor is highly expressed in the magnum and liver followed by the uterus, which secrete egg white, egg yolk, and eggshell precursors, respectively. The results also revealed that ovoinhibitor expression is increased in the liver during sexual maturation followed by a subsequent decrease in mature hens. Ovoinhibitor was purified from the egg yolk plasma from nonfertilized eggs using two consecutive affinity chromatographies and gel filtration. Purified egg yolk ovoinhibitor was shown to inhibit trypsin and subtilisin. It was shown that purified egg yolk ovoinhibitor exhibited antimicrobial activities against Bacillus thuringiensis. The results suggest that this anti-protease plays a significant role in antibacterial egg defense against Bacillus spp., preventing contamination of table eggs (nonfertilized eggs) and protecting the chick embryo (fertilized eggs).
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Dates et versions

hal-01129593 , version 1 (10-03-2015)

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Marie-Christine Bourin, Joël Gautron, Magali Berges, S. Attucci, Gwenaelle Le Blay, et al.. Antimicrobial potential of egg Yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg. Journal of Agricultural and Food Chemistry, 2011, 59 (23), pp.12368-12374. ⟨10.1021/jf203339t⟩. ⟨hal-01129593⟩
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