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Article Dans Une Revue Journal of materials chemistry‎ B Année : 2013

Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films

Résumé

A novel method, based on the electrochemical manipulation of the sol-gel process, was developed to immobilize bacteria in thin hybrid sol-gel films. This enabled the safe immobilization of Escherichia coli on electrode surfaces. E. coli strains C600, MG1655 pUCD607 and MG1655 pZNTA-GFP were incorporated and physically encapsulated in a hybrid sol-gel matrix and the metabolic activity and membrane integrity of the bacteria were assessed as a function of the aging time in the absence of nutrients at +4 degrees C or -80 degrees C. LIVE/DEAD BacLight bacterial viability analysis detected by epifluorescence microscopy indicated the preservation of 95% of E. coli C600 membrane integrity in the sol-gel film. The presence of chitosan, trehalose and polyethylene glycol additives was shown to strongly improve the viability of E. coli cells in the electrodeposited matrix for 1 month after encapsulation. Finally, the bioluminescent activity of E. coli MG1655 pUCD607 was preserved by approximately 50% of the cells present in such composite films.
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Dates et versions

hal-01115124 , version 1 (10-02-2015)

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Wissam Ghach, Mathieu Etienne, Patrick Billard, Frédéric P. A. Jorand, Alain Walcarius. Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films. Journal of materials chemistry‎ B, 2013, 1 (7), pp.1052-1059. ⟨10.1039/c2tb00421f⟩. ⟨hal-01115124⟩
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