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Article Dans Une Revue Analytica Chimica Acta Année : 2014

Direct TLC/MALDI–MS coupling for modified polyamidoamine dendrimers analyses

Résumé

Polyamidoamine (PAMAM) are synthetic dendrimers which present attractive properties for the biolog- ical and biomedical fields, as they proved to be efficient drug and gene carriers. In order to increase their transfection efficiency, chemical modifications of the amino end-groups had been reported. In this work, the synthesis of the ammonia-cored G1(N) PAMAM and the consecutive chemical modification with glycine or phenylalanine amino-acids were monitored using the coupling of thin layer chromatography (TLC) with matrix–assisted laser desorption ionization–mass spectrometry (MALDI–MS). Thus, the moni- toring of the PAMAM synthesis included the identification of the by-products such as defective structures of PAMAM dendrimers as well as the study of phenylalanine-grafted PAMAM oligomer distribution.
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Dates et versions

hal-01107942 , version 1 (21-01-2015)

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Emma-Dune Leriche, Marie Hubert-Roux, Martin C. Grossel, Catherine M. Lange, Carlos Afonso, et al.. Direct TLC/MALDI–MS coupling for modified polyamidoamine dendrimers analyses. Analytica Chimica Acta, 2014, 808, pp.144-150. ⟨10.1016/j.aca.2013.09.037⟩. ⟨hal-01107942⟩
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