Agar-supported cultivation of Halorubrum sp. SSR, and production of halocin C8 on the scale-up prototype Platotex. - Archive ouverte HAL
Article Dans Une Revue Extremophiles Année : 2014

Agar-supported cultivation of Halorubrum sp. SSR, and production of halocin C8 on the scale-up prototype Platotex.

Résumé

Halorubrum sp. SSR was isolated from a solar saltern in Algeria. The strain exhibited a high antibiotic activity against the indicator strain Natronorubrum aibiense G23, and the bioactive compound showed thermal, acid and alkali stability. SSR was grown on agar-supported cultivation (AgSF) to compare yields and applicability with traditional submerged cultivation. AgSF scale-up was implemented taking benefit from the solid-state cultivation prototype Platotex. This technology leads to high amounts of the target Halocin and facilitate the downstream steps. The antibiotic compound was purified according to a fast efficient procedure including ion exchange chromatography followed by a fractionation on C18 Sep-Pack cartridge. The compound was identified as Halocin C8 according to N-terminal amino acid sequencing and high-resolution mass spectrometry.

Domaines

Chimie
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Dates et versions

hal-01102307 , version 1 (12-01-2015)

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Citer

Rima Meknaci, Philippe Lopes, Claudine Servy, Jean-Pierre Le Caer, Jean-Pierre Andrieu, et al.. Agar-supported cultivation of Halorubrum sp. SSR, and production of halocin C8 on the scale-up prototype Platotex.. Extremophiles, 2014, 18 (6), pp.1049-1055. ⟨10.1007/s00792-014-0682-5⟩. ⟨hal-01102307⟩
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