Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta Crystallographica Section F: Structural Biology and Crystallization Communications Année : 2014

Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.

Résumé

The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 Å resolution, crystals of gp34(781-1289) diffracting to 1.9 Å resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.
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Dates et versions

hal-01100474 , version 1 (24-11-2020)

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Meritxell Granell, Mikiyoshi Namura, Sara Alvira, Carmela Garcia-Doval, Abhimanyu K Singh, et al.. Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2014, pp.970-5. ⟨10.1107/S2053230X14010449⟩. ⟨hal-01100474⟩
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