The age and genomic integrity of neurons after cortical stroke in humans.
Hagen B Huttner
(1)
,
Olaf Bergmann
(2)
,
Mehran Salehpour
(3)
,
Attila Rácz
(4)
,
Jemal Tatarishvili
(5)
,
Emma Lindgren
(6)
,
Tamás Csonka
(7)
,
László Csiba
(7)
,
Tibor Hortobágyi
(8)
,
Gábor Méhes
(7)
,
Elisabet Englund
(9)
,
Beata Werne Solnestam
(10)
,
Sofia Zdunek
(2)
,
Christian Scharenberg
(11)
,
Lena Ström
(12)
,
Patrik Ståhl
(1)
,
Benjamin Sigurgeirsson
(10)
,
Andreas Dahl
(13)
,
Stefan Schwab
(14)
,
Göran Possnert
(15)
,
Samuel Bernard
(16, 17, 18)
,
Zaal Kokaia
(5)
,
Olle Lindvall
(5)
,
Joakim Lundeberg
(10)
,
Jonas Frisén
(2)
1
Karolinska Institutet [Stockholm]
2 Department of Cell and Molecular Biology [Uppsala]
3 Department of Physics and Astronomy [Uppsala]
4 FAU - Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg
5 Lund Stem Cell Center
6 Department of Mathematical Sciences
7 University of Debrecen
8 Structural Studies
9 Skane University Hospital [Lund]
10 KTH - KTH Royal Institute of Technology [Stockholm]
11 Karolinska University Hospital [Stockholm]
12 Department of Cell and Molecular Biology [Stockholm]
13 MSSMat - Laboratoire de mécanique des sols, structures et matériaux
14 ELCF - École de Langue et de Civilisation Françaises, Université de Genève
15 Angström Laboratory
16 DRACULA - Multi-scale modelling of cell dynamics : application to hematopoiesis
17 MMCS - Modélisation mathématique, calcul scientifique
18 ICJ - Institut Camille Jordan
2 Department of Cell and Molecular Biology [Uppsala]
3 Department of Physics and Astronomy [Uppsala]
4 FAU - Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg
5 Lund Stem Cell Center
6 Department of Mathematical Sciences
7 University of Debrecen
8 Structural Studies
9 Skane University Hospital [Lund]
10 KTH - KTH Royal Institute of Technology [Stockholm]
11 Karolinska University Hospital [Stockholm]
12 Department of Cell and Molecular Biology [Stockholm]
13 MSSMat - Laboratoire de mécanique des sols, structures et matériaux
14 ELCF - École de Langue et de Civilisation Françaises, Université de Genève
15 Angström Laboratory
16 DRACULA - Multi-scale modelling of cell dynamics : application to hematopoiesis
17 MMCS - Modélisation mathématique, calcul scientifique
18 ICJ - Institut Camille Jordan
Samuel Bernard
- Fonction : Auteur
- PersonId : 1590
- IdHAL : samuel-bernard
Joakim Lundeberg
- Fonction : Auteur
- PersonId : 772799
- ORCID : 0000-0003-4313-1601
Résumé
It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival.