Techniques that enable user interaction with a mixed environment in natural way and low-cost may provide a great potential to increase the degree of virtual presence. In this paper, we present a low-cost 3D interaction technique based on ARToolKit planar marker in order to interact with mixed reality environment, and manipulate 3D virtual object. This proposed method that we called “2 in 1 Marker” offers a six-degree of freedom, and it allows the user to perform different interaction tasks. An evaluation study of the proposed interaction technique is given in this paper to evaluate the user performance using wearable and handheld devices.