Effect of C5-Methylation of Cytosine on the Photoreactivity of DNA: A Joint Experimental and Computational Study of TCG Trinucleotides - Archive ouverte HAL
Article Dans Une Revue Journal of the American Chemical Society Année : 2014

Effect of C5-Methylation of Cytosine on the Photoreactivity of DNA: A Joint Experimental and Computational Study of TCG Trinucleotides

Akos Banyasz
Thierry Douki
Dimitra Markovitsi

Résumé

DNA methylation, occurring at the 5 position of cytosine, is a natural process associated with mutational hotspots in skin tumors. By combining experimental techniques (optical spectroscopy, HPLC coupled to mass spectrometry) with theoretical methods (molecular dynamics, DFT/TD-DFT calculations in solution), we study trinucleotides with key sequences (TCG/T5mCG) in the UV-induced DNA damage. We show how the extra methyl, affecting the conformational equilibria and, hence, the electronic excited states, increases the quantum yield for the formation of cyclobutane dimers while reducing that of (6-4) adducts.
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Dates et versions

hal-01075288 , version 1 (17-10-2014)

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Luciana Esposito, Akos Banyasz, Thierry Douki, Marion Perron, Dimitra Markovitsi, et al.. Effect of C5-Methylation of Cytosine on the Photoreactivity of DNA: A Joint Experimental and Computational Study of TCG Trinucleotides. Journal of the American Chemical Society, 2014, 136 (31), pp.10838-10841. ⟨10.1021/ja5040478⟩. ⟨hal-01075288⟩
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