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Article Dans Une Revue Journal of visualized experiments : JoVE Année : 2014

Electronic tongue generating continuous recognition patterns for protein analysis.

Résumé

In current protocol, a combinatorial approach has been developed to simplify the design and production of sensing materials for the construction of electronic tongues (eT) for protein analysis. By mixing a small number of simple and easily accessible molecules with different physicochemical properties, used as building blocks (BBs), in varying and controlled proportions and allowing the mixtures to self-assemble on the gold surface of a prism, an array of combinatorial surfaces featuring appropriate properties for protein sensing was created. In this way, a great number of cross-reactive receptors can be rapidly and efficiently obtained. By combining such an array of combinatorial cross-reactive receptors (CoCRRs) with an optical detection system such as surface plasmon resonance imaging (SPRi), the obtained eT can monitor the binding events in real-time and generate continuous recognition patterns including 2D continuous evolution profile (CEP) and 3D continuous evolution landscape (CEL) for samples in liquid. Such an eT system is efficient for discrimination of common purified proteins.

Dates et versions

hal-01072629 , version 1 (08-10-2014)

Identifiants

Citer

Yanxia Hou, Maria Genua, Laurie-Amandine Garçon, Arnaud Buhot, Roberto Calemczuk, et al.. Electronic tongue generating continuous recognition patterns for protein analysis.. Journal of visualized experiments : JoVE, 2014, 91, pp.DOI 10.3791/51901. ⟨10.3791/51901⟩. ⟨hal-01072629⟩
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