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Article Dans Une Revue Optics Express Année : 2013

High countrate real-time FCS using F2Cor.

Emmanuel Schaub

Résumé

We present a fluorescence correlation spectroscopy setup based on a software correlator. The setup can process autocorrelation curves in real-time at countrate as high as 8MHz, with time resoln. of 1μs. It uses the F2Cor autocorrelation algorithm, a low cost counting board and a desktop computer. Sym. normalization, which improves the signal to noise ratio of the FCS curve for large values of the lag-time, is adapted to the F2Cor algorithm. A new acquisition mode, which we call oscilloscope-mode, is presented. It takes advantage of the flexibility F2Cor, and proves to be very useful for optical setup adjustment. As an application of this setup, we performed FCS measurements on a ref. tetramethylrhodamine soln. at high concn., up to 2.5μM, which extend to the micromolar range the concn. applicable in FCS, using a conventional optical setup. At such high countrates the FCS curves need to be cor. for dead-time of the photo-detector, which was done successfully. [on SciFinder(R)]

Dates et versions

hal-01072049 , version 1 (07-10-2014)

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Emmanuel Schaub. High countrate real-time FCS using F2Cor.. Optics Express, 2013, 21, pp.23543--23555. ⟨10.1364/OE.21.023543⟩. ⟨hal-01072049⟩
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