Numerous chemical products are dispersed into the environment, and the consequences can be sometimes harmful to humans and ecosystems. Pharmaceutical compounds and hormone steroids are among these substances that concern the scientific community. Currently, little data are available on the presence and fate of these compounds in the environment and, in particular, for solid matrices. Therefore, the aim of this work was to perform soil column experiments to evaluate the accumulation, transfer and degradation of these substances in soil. The analyses were based on efficient sample preparation followed by sensitive and selective liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). For this purpose, 23 compounds were chosen including both pharmaceutical compounds as well as steroid hormones. In addition, this experiment was performed on two soils with different properties (% clay, pH, etc.). To the best of our knowledge, no soil column experiments have been performed previously on a large number of pharmaceutical compounds and steroid hormones. Significant transfer was observed only for sulphonamides that can be justified by their polarity (log K ow < 3). Furthermore, some compounds have a cationic characteristic and are likely to be not much mobiles in soil due to cation exchange process. However, it was observed that the migration of the substances depends on the soil characteristics, such as the amount of clay and the pH values. Regarding the degradation, it was noticed that substances degraded rapidly in the two soils. Indeed, for most substances, their half-lives were lower than 20 days. Furthermore, it was observed that the degradation rate depended on the soil.