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Article Dans Une Revue Applied Physics Letters Année : 2014

Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays

P. Ghenuche
  • Fonction : Auteur
J. de Torres
  • Fonction : Auteur
Patrick Ferrand
Jérôme Wenger

Résumé

Fluorescence sensing and fluorescence correlation spectroscopy (FCS) are powerful methods to detect and characterize single molecules; yet, their use has been restricted by expensive and complex optical apparatus. Here, we present a simple integrated design using a self-assembled bidimensional array of microspheres to realize multi-focus parallel detection scheme for FCS. We simultaneously illuminate and collect the fluorescence from several tens of microspheres, which all generate their own photonic nanojet to efficiently excite the molecules and collect the fluorescence emission. Each photonic nanojet contributes to the global detection volume, reaching FCS detection volumes of several tens of femtoliters while preserving the fluorescence excitation and collection efficiencies. The microspheres photonic nanojets array enables FCS experiments at low picomolar concentrations with a drastic reduction in apparatus cost and alignment constraints, ideal for microfluidic chip integration.
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Dates et versions

hal-01069861 , version 1 (30-09-2014)

Identifiants

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P. Ghenuche, J. de Torres, Patrick Ferrand, Jérôme Wenger. Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays. Applied Physics Letters, 2014, 105 (13), pp.131102. ⟨10.1063/1.4896852⟩. ⟨hal-01069861⟩
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