A 'click' chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Organic & Biomolecular Chemistry Année : 2014

A 'click' chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins

Y. Wang
  • Fonction : Auteur
Z. Assaf
  • Fonction : Auteur
F. Ziarelli
  • Fonction : Auteur
A. Latifi
  • Fonction : Auteur
O. Lamrabet
  • Fonction : Auteur
F. Qu
  • Fonction : Auteur
C-C. Zhang
  • Fonction : Auteur

Résumé

An ingenious and specific affinity resin designed to capture the 2-oxoglutaric acid (2-OG) binding proteins was constructed by appending a 2-OG tag to the solid resin via a Cu-catalyzed Huisgen "click" reaction. The so-obtained affinity resin was able to recognize, retain and separate the established 2-OG binding protein NtcA in both the pure form and crude cellular extract, thus constituting a valuable means of searching for novel 2-OG receptors with a view to exploring the signalling pathways of 2-OG, a key Krebs cycle intermediate with unprecedented signalling functions.

Domaines

Chimie

Dates et versions

hal-01067964 , version 1 (24-09-2014)

Identifiants

Citer

Y. Wang, Z. Assaf, X. Liu, F. Ziarelli, A. Latifi, et al.. A 'click' chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins. Organic & Biomolecular Chemistry, 2014, 12 (33), pp.6470-6475. ⟨10.1039/c4ob01005a⟩. ⟨hal-01067964⟩
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