In this paper, we introduce a general framework for waveform design and signal processing, dedicated to the study of turbulent flow phenomena. In a bi-static configuration, by transmitting a specific waveform with a predefined instantaneous frequency law (IFL), within the bounds of the Kolmogorov spectrum, the turbulent media will modify the IFL at the receiving side. We propose a new methodology to estimate this change and to exploit it for velocity estimation using acoustic signals. In this way, the amplitude based velocity estimation techniques can be substituted by non stationary time - frequency signal processing. This technique proves to be more robust in terms of interferences and can provide a more detailed representation of any turbulent environment.