Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2014

Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology

Résumé

Intracellular targeting of mRNAs has recently emerged as a prevalent mechanism to control protein localization. For mitochondria, a cotranslational model of protein import is now proposed in parallel to the conventional posttranslational model, and mitochondrial targeting of mRNAs has been demonstrated in various organisms. Voltage-dependent anion channels (VDACs) are the most abundant proteins in the outer mitochondrial membrane and the major transport pathway for numerous metabolites. Four nucleus-encoded VDACs have been identified in Arabidopsis thaliana. Alternative cleavage and polyadenylation generate two VDAC3 mRNA isoforms differing by their 3' UTR. By using quantitative RT-PCR and in vivo mRNA visualization approaches, the two mRNA variants were shown differentially associated with mitochondria. The longest mRNA presents a 3' extension named alternative UTR (aUTR) that is necessary and sufficient to target VDAC3 mRNA to the mitochondrial surface. Moreover, aUTR is sufficient for the mitochondrial targeting of a reporter transcript, and can be used as a tool to target an unrelated mRNA to the mitochondrial surface. Finally, VDAC3-aUTR mRNA variant impacts mitochondria morphology and size, demonstrating the role of mRNA targeting in mitochondria biogenesis.

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hal-01050870 , version 1 (25-07-2014)

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Morgane Michaud, Elodie Ubrig, Sophie Filleur, Mathieu Erhardt, Geneviève Ephritikhine, et al.. Differential targeting of VDAC3 mRNA isoforms influences mitochondria morphology. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (24), pp.8991-8996. ⟨10.1073/pnas.1402588111⟩. ⟨hal-01050870⟩
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