SHOOT GROWTH1 Maintains Arabidopsis Epigenomes by Regulating IBM1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2014

SHOOT GROWTH1 Maintains Arabidopsis Epigenomes by Regulating IBM1

Vincent Coustham
Isabelle Gy
  • Fonction : Auteur
  • PersonId : 1204769
Francisco Cubillos
  • Fonction : Auteur
  • PersonId : 937964
Envel Kerdaffrec
  • Fonction : Auteur
  • PersonId : 958105
Olivier Loudet
Nicolas Bouché

Résumé

Maintaining correct DNA and histone methylation patterns is essential for the development of all eukaryotes. In Arabidopsis, we identified SHOOT GROWTH1 (SG1), a novel protein involved in the control of gene methylation. SG1 contains both a Bromo-Adjacent Homology (BAH) domain found in several chromatin regulators and an RNA-Recognition Motif (RRM). The sg1 mutations are associated with drastic pleiotropic phenotypes. The mutants degenerate after few generations and are similar to mutants of the histone demethylase INCREASE IN BONSAI METHYLATION1 (IBM1). A methylome analysis of sg1 mutants revealed a large number of gene bodies hypermethylated in the cytosine CHG context, associated with an increase in di-methylation of lysine 9 on histone H3 tail (H3K9me2), an epigenetic mark normally found in silenced transposons. The sg1 phenotype is suppressed by mutations in genes encoding the DNA methyltransferase CHROMOMETHYLASE3 (CMT3) or the histone methyltransferase KRYPTONITE (KYP), indicating that SG1 functions antagonistically to CMT3 or KYP. We further show that the IBM1 transcript is not correctly processed in sg1, and that the functional IBM1 transcript complements sg1. Altogether, our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1.

Mots clés

Fichier principal
Vignette du fichier
2014_Coustham_Plos_One_1.pdf (7.43 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01019546 , version 1 (27-05-2020)

Identifiants

Citer

Vincent Coustham, Daniela Loredan Vlad, Aurélie Deremetz, Isabelle Gy, Francisco Cubillos, et al.. SHOOT GROWTH1 Maintains Arabidopsis Epigenomes by Regulating IBM1. PLoS ONE, 2014, 9, online (1), Non paginé. ⟨10.1371/journal.pone.0084687⟩. ⟨hal-01019546⟩
84 Consultations
20 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More